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human oral squamous carcinoma cells scc 4  (ATCC)


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    ATCC human oral squamous carcinoma cells scc 4
    Human Oral Squamous Carcinoma Cells Scc 4, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 751 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+oral+squamous+carcinoma+cells+scc+4/SCC-4/pm41186846-115-9-15
    Average 96 stars, based on 751 article reviews
    human oral squamous carcinoma cells scc 4 - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    other:

    Article Title: Combining NAD(P)H-FLIM and PLIM of Ir5 pH sensor to optimize photodynamic therapy through metabolic intracellular pattern segmentation.
    Article Snippet: We performed this study in two different cell lines, Human oral squamous carcinoma cells SCC-4 (ATCC-No. 1 3 All hypothesis tests were performed using a significance level of 5% (p = 0.05).

    Modification:

    Article Title: A biocompatible phosphorescent Ir(iii) oxygen sensor functionalized with oligo(ethylene glycol) groups: synthesis, photophysics and application in PLIM experiments
    Article Snippet: Phosphorescent transition metal complexes with emission in near infrared (NIR) area and appreciable quantum yield, compatible with physiological media and suitable for application as effective oxygen sensors in biological systems are still a challenge for synthetic organometallic chemistry.. In addition to the sufficiently high requirements in photophysics, they also have to meet strict conditions for biocompatibility and selectivity of sensory response in a multicomponent biological medium.. Building on our previous research we have synthesized three novel iridium [Ir(N^C)2(N^N)] complexes (N^C – metalated ligands based on benzothienyl-phenanthridine core; N^N – pyridine-triazole diimine chelate), with the periphery decorated with 1, 2 or 3 relatively small “double-tail” oligo(ethylene glycol) (OEG) {C(O)NHCH(CH2OC2H4OC2H4OCH3)2} functions.



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    human oral squamous carcinoma cells scc 4  (ATCC)
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    human oral squamous cell carcinoma line scc4  (ATCC)
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    Characterization of <t>SCC4-EGFP</t> spheroids with different volumes. (A) Structure of the dual-function antibody conjugate. The number of BPD and SiNC(OH) molecules per antibody molecule are denoted by “ x ” and “ y ”, respectively. (B) Graph representing the change in SCC4-EGFP spheroid volume on different days, post cell-seeding. (C) Representative images of SCC4-EGFP 3D spheroids formed with different cell numbers (2500, 5000, 10,000, and 25,000 cells). Cell numbers are mentioned at the top, while spheroid volumes are mentioned within the images (scale bar = 500 μm).
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    human oral squamous cell carcinoma cell lines scc 4  (ATCC)
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    Characterization of <t>SCC4-EGFP</t> spheroids with different volumes. (A) Structure of the dual-function antibody conjugate. The number of BPD and SiNC(OH) molecules per antibody molecule are denoted by “ x ” and “ y ”, respectively. (B) Graph representing the change in SCC4-EGFP spheroid volume on different days, post cell-seeding. (C) Representative images of SCC4-EGFP 3D spheroids formed with different cell numbers (2500, 5000, 10,000, and 25,000 cells). Cell numbers are mentioned at the top, while spheroid volumes are mentioned within the images (scale bar = 500 μm).
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    Characterization of SCC4-EGFP spheroids with different volumes. (A) Structure of the dual-function antibody conjugate. The number of BPD and SiNC(OH) molecules per antibody molecule are denoted by “ x ” and “ y ”, respectively. (B) Graph representing the change in SCC4-EGFP spheroid volume on different days, post cell-seeding. (C) Representative images of SCC4-EGFP 3D spheroids formed with different cell numbers (2500, 5000, 10,000, and 25,000 cells). Cell numbers are mentioned at the top, while spheroid volumes are mentioned within the images (scale bar = 500 μm).

    Journal: Bioconjugate Chemistry

    Article Title: Dual-Function Antibody Conjugate-Enabled Photoimmunotherapy Complements Fluorescence and Photoacoustic Imaging of Head and Neck Cancer Spheroids

    doi: 10.1021/acs.bioconjchem.3c00406

    Figure Lengend Snippet: Characterization of SCC4-EGFP spheroids with different volumes. (A) Structure of the dual-function antibody conjugate. The number of BPD and SiNC(OH) molecules per antibody molecule are denoted by “ x ” and “ y ”, respectively. (B) Graph representing the change in SCC4-EGFP spheroid volume on different days, post cell-seeding. (C) Representative images of SCC4-EGFP 3D spheroids formed with different cell numbers (2500, 5000, 10,000, and 25,000 cells). Cell numbers are mentioned at the top, while spheroid volumes are mentioned within the images (scale bar = 500 μm).

    Article Snippet: Human oral squamous cell carcinoma line SCC4 was obtained from American Type Culture Collection (ATCC CRL-1624, Manassas, VA).

    Techniques:

    Fluorescence imaging of DFAC-treated SCC4 spheroids. (A) Experimental scheme of SCC4-EGFP 3D spheroid treatment with DFAC and imaging. (B) IVIS fluorescence imaging of DFAC-treated SCC4 spheroids prepared as gelatin-embedded tissue-mimicking phantoms in a 96-well plate (scale bar = 2.5 mm). Cell numbers and volumes are mentioned at the top, while DFAC concentrations (SiNC(OH) equivalent) are mentioned on the left. (C) Quantification of fluorescence signals from the IVIS images is represented as the tumor spheroid-to-background ratio. Data are presented as mean ± SD ( n ≥ 3), analyzed using one-way ANOVA with Tukey’s test for post hoc analysis. p -Values <0.05 were considered to be significant and are indicated by asterisks as follows: ns p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.

    Journal: Bioconjugate Chemistry

    Article Title: Dual-Function Antibody Conjugate-Enabled Photoimmunotherapy Complements Fluorescence and Photoacoustic Imaging of Head and Neck Cancer Spheroids

    doi: 10.1021/acs.bioconjchem.3c00406

    Figure Lengend Snippet: Fluorescence imaging of DFAC-treated SCC4 spheroids. (A) Experimental scheme of SCC4-EGFP 3D spheroid treatment with DFAC and imaging. (B) IVIS fluorescence imaging of DFAC-treated SCC4 spheroids prepared as gelatin-embedded tissue-mimicking phantoms in a 96-well plate (scale bar = 2.5 mm). Cell numbers and volumes are mentioned at the top, while DFAC concentrations (SiNC(OH) equivalent) are mentioned on the left. (C) Quantification of fluorescence signals from the IVIS images is represented as the tumor spheroid-to-background ratio. Data are presented as mean ± SD ( n ≥ 3), analyzed using one-way ANOVA with Tukey’s test for post hoc analysis. p -Values <0.05 were considered to be significant and are indicated by asterisks as follows: ns p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.

    Article Snippet: Human oral squamous cell carcinoma line SCC4 was obtained from American Type Culture Collection (ATCC CRL-1624, Manassas, VA).

    Techniques: Fluorescence, Imaging

    Photoacoustic imaging of DFAC-treated SCC4 spheroids. (A) Photoacoustic imaging of DFAC-treated SCC4 spheroids prepared as gelatin-embedded tissue-mimicking phantoms in a 96-well plate (scale bar = 2.5 mm). Cell numbers and volumes are mentioned on the left, while DFAC concentrations (SiNC(OH) equivalent) are mentioned on the top. (B) Quantification of photoacoustic signals from the images represented as the normalized photoacoustic signal intensity per mm 3 . Data are presented as mean ± SD ( n ≥ 3), analyzed using one-way Brown–Forsythe and Welch ANOVA test with post hoc analysis. p -Values <0.05 were considered to be significant and are indicated by asterisks as follows: ns p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.

    Journal: Bioconjugate Chemistry

    Article Title: Dual-Function Antibody Conjugate-Enabled Photoimmunotherapy Complements Fluorescence and Photoacoustic Imaging of Head and Neck Cancer Spheroids

    doi: 10.1021/acs.bioconjchem.3c00406

    Figure Lengend Snippet: Photoacoustic imaging of DFAC-treated SCC4 spheroids. (A) Photoacoustic imaging of DFAC-treated SCC4 spheroids prepared as gelatin-embedded tissue-mimicking phantoms in a 96-well plate (scale bar = 2.5 mm). Cell numbers and volumes are mentioned on the left, while DFAC concentrations (SiNC(OH) equivalent) are mentioned on the top. (B) Quantification of photoacoustic signals from the images represented as the normalized photoacoustic signal intensity per mm 3 . Data are presented as mean ± SD ( n ≥ 3), analyzed using one-way Brown–Forsythe and Welch ANOVA test with post hoc analysis. p -Values <0.05 were considered to be significant and are indicated by asterisks as follows: ns p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.

    Article Snippet: Human oral squamous cell carcinoma line SCC4 was obtained from American Type Culture Collection (ATCC CRL-1624, Manassas, VA).

    Techniques: Imaging

    Long-term response of SCC4 spheroids to DFAC-mediated PIT. (top) Experimental schedule for the long-term response of SCC4 spheroids to PIT. Cells were seeded in CellCarrier Spheroid ULA 96-well microplates, followed by incubation with DFAC for 24 h. The spheroids were then irradiated with varying light doses of 10–100 J/cm 2 , followed by imaging for 5 weeks. (bottom left) Maximum intensity projection (MIP) of fluorescence signals from SCC4 spheroids on day 31 post-PDT. Scale bar corresponds to 500 μm. (bottom right) Relative viability of SCC4 spheroids treated with PIT is shown in the right panel.

    Journal: Bioconjugate Chemistry

    Article Title: Dual-Function Antibody Conjugate-Enabled Photoimmunotherapy Complements Fluorescence and Photoacoustic Imaging of Head and Neck Cancer Spheroids

    doi: 10.1021/acs.bioconjchem.3c00406

    Figure Lengend Snippet: Long-term response of SCC4 spheroids to DFAC-mediated PIT. (top) Experimental schedule for the long-term response of SCC4 spheroids to PIT. Cells were seeded in CellCarrier Spheroid ULA 96-well microplates, followed by incubation with DFAC for 24 h. The spheroids were then irradiated with varying light doses of 10–100 J/cm 2 , followed by imaging for 5 weeks. (bottom left) Maximum intensity projection (MIP) of fluorescence signals from SCC4 spheroids on day 31 post-PDT. Scale bar corresponds to 500 μm. (bottom right) Relative viability of SCC4 spheroids treated with PIT is shown in the right panel.

    Article Snippet: Human oral squamous cell carcinoma line SCC4 was obtained from American Type Culture Collection (ATCC CRL-1624, Manassas, VA).

    Techniques: Incubation, Irradiation, Imaging, Fluorescence

    Recurrence of SCC4 spheroid growth after DFAC-mediated PIT. SCC4 spheroids with different volumes were treated with PIT with different light doses: (A) 5 J/cm 2 , (B) 25 J/cm 2 , (C) 50 J/cm 2 , and (D) 100 J/cm 2 .

    Journal: Bioconjugate Chemistry

    Article Title: Dual-Function Antibody Conjugate-Enabled Photoimmunotherapy Complements Fluorescence and Photoacoustic Imaging of Head and Neck Cancer Spheroids

    doi: 10.1021/acs.bioconjchem.3c00406

    Figure Lengend Snippet: Recurrence of SCC4 spheroid growth after DFAC-mediated PIT. SCC4 spheroids with different volumes were treated with PIT with different light doses: (A) 5 J/cm 2 , (B) 25 J/cm 2 , (C) 50 J/cm 2 , and (D) 100 J/cm 2 .

    Article Snippet: Human oral squamous cell carcinoma line SCC4 was obtained from American Type Culture Collection (ATCC CRL-1624, Manassas, VA).

    Techniques: